Cover Picture: One-Pot Chemoenzymatic Cascade for Labeling of the Epigenetic Marker 5-Hydroxymethylcytosine (ChemBioChem 13/2015)
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چکیده
منابع مشابه
One-pot chemoenzymatic synthesis of trolline and tetrahydroisoquinoline analogues
Chemoenzymatic reaction cascades can provide access to chiral compounds from low-cost starting materials in one pot. Here we describe one-pot asymmetric routes to tetrahydroisoquinoline alkaloids (THIAs) using the Pictet–Spenglerase norcoclaurine synthase (NCS) followed by a cyclisation, to give alkaloids with two new heterocyclic rings. These reactions operated with a high atom economy to gene...
متن کاملOne-pot chemoenzymatic multicomponent synthesis of thiazole derivatives.
A novel chemoenzymatic one-pot multicomponent synthesis of thiazole derivatives was developed. A series of thiazole derivatives were synthesized with high yields up to 94% under mild enzyme-catalyzed conditions. The blank and control experiments reveal that trypsin from porcine pancreas (PPT) displayed great catalytic activity to promote this reaction and showed a wide tolerance range towards d...
متن کاملCover Picture: Detection of mRNA in Mammalian Cells with a Split Ribozyme Reporter (ChemBioChem 6/2006)
The cover picture shows a split RNA reporter strategy for detecting target mRNA in mammalian cells. A tetrahymena group I intron ribozyme reporter is split into two halves, each of which carries a part of the coding sequence of the reporter mRNA, a fragment of the ribozyme, and an antisense sequence complementary to a target mRNA. Both halves bind with the target mRNA and are assembled into an ...
متن کامل5-Hydroxymethylcytosine: a new kid on the epigenetic block?
The discovery of the Ten-Eleven-Translocation (TET) oxygenases that catalyze the hydroxylation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) has triggered an avalanche of studies aiming to resolve the role of 5hmC in gene regulation if any. Hitherto, TET1 is reported to bind to CpG-island (CGI) and bivalent promoters in mouse embryonic stem cells, whereas binding at DNAseI hyperse...
متن کاملLoss of 5-Hydroxymethylcytosine Is an Epigenetic Hallmark of Melanoma
DNA methylation at the 5 position of cytosine (5-mC) is a key epigenetic mark that is critical for various biological and pathological processes. 5-mC can be converted to 5-hydroxymethylcytosine (5-hmC) by the ten-eleven translocation (TET) family of DNA hydroxylases. Here, we report that "loss of 5-hmC" is an epigenetic hallmark of melanoma, with diagnostic and prognostic implications. Genome-...
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ژورنال
عنوان ژورنال: ChemBioChem
سال: 2015
ISSN: 1439-4227
DOI: 10.1002/cbic.201581301